top of page

CALL US TODAY FOR AN APPOINTMENT

Creative Animodel

45-1 Ramsey Road, Shirley, NY, 11967, USA

Mailed: info@Creative-Animodel.com

Contact No: + 1-631-626-9181 or + 44-207-048-3343

Website: http://www.creative-animodel.com

 

THE DEVELOPMENT OF THE CONDITIONAL KNOCKOUT MICE

 

Development of novel therapies has always been an issue of great concern. It is never easy and has a very high rate of unsuccessful results. Keeping in mind the difficulty of the task at hand, several services have sprung up in order or an efficient development as well as in order to accelerate the pace at which the answer to these various problems need to be found. So far, the services are doing a great job and with their hard work, they have successfully developed a number of novel therapies.

 

These services have a team of very well experienced preclinical units who conduct a number of toxicology, PK and PD experiments for the evaluation of novel compounds. In all their services, the generation of conditional knockout mice has been an immense feat. This generation is brought about by beginning from similar targeted clones of ES cells. The entire process also reduces the several risks that are usually associated with the constitutive knockouts which include situations like complex phenotype and the lethality of the entire process. They also have a reduced risk of the other constituent knockout associated risks by allowing a switch that the conditional models might require.

 

Under this topic of discussion, mention should be made of animodel which have had a significant achievement over the past few years. Everything about us including our structure of experimenting as well as the tools needed for experiment are given special attention that makes our other experimentation a success in most cases.

 

We strategize the entire knockout system in a way that allows us the maximum observation thus helping us rule out ingredients of failure in our experimentation. We pride ourselves in finding out approaches that make sure to not be time consuming and facilitate the up-grading of models allowing a higher rate of positive results. For more information visit our website: www.creative-animodel.com

RNAi Gene Knockdown Services by Creative Animodel

Creative Animodel takes up long time challenges to develop therapies for chronic and long-time illness. The company is an experience life science CRO and offer accelerated preclinical services, by using a number of bio-techniques to know more about the disease and how to combat it. RNAi Gene knockdown experiments have become very popular in the world of medical science. It has become and common and routine function to assess and study the gene functions in a quick and easy way. A very widely used approach in performing gene knockdown experiments is the RNA interference. The process is to conduct experiments on cell cultures and transgenic animals.

 

Creating mice with down regulated target genes involves a number of purposes. The rapid generation of the mice is done by the transduction of early embryos with adenoviral vectors and lent viral silencing. Such methodologies open up chances of large scale generation of knockdown transgenic mice, which can be used for functional genomics studies. Within just 7 seven weeks, this process enables the production of transgenic mice.

 

RNAi gene knockdown is a method which is being employed by scientists in different parts of the globe, and is yielding fruitful results. Creative Animodel, conducts all these experiments in absolute propriety. Their RNAi technology can help you in inducing and reversing ubiquitous gene knockdown in mice at different given times. There customer services, will be happy to help you and answer all your queries in detail. You can give them a call at any time of the day and any day of the week.

Get expert toxicology service in the US

As daily human interaction with all that is artificially manufactured reaches an all time high, the study of the various chemicals that make up everyday objects, and how they affect natural organisms has gained more prominence than ever. The field of toxicology has evolved immensely in the past few decades, all thanks to the amazing leaps that science has made in terms of equipments and technologies that can be used to detect and analyze chemicals, even when present in trace amounts. The field requires an extensive understanding of chemistry and biology, as well as a thorough comprehension of how the two field mix.

 

Secure reliable services for toxicology today

 

The study of toxins and chemicals takes many forms. Toxicity can be introduced by a sudden large dose of a harmful chemical, and can also be caused due to slow accumulation. DDT is a prime example of slow accumulation and was banned in virtually every developed country post the discovery that the chemical ended up in animals living as far away as the polar caps. In total there are 4 specific types of toxicity:

 

  • Acute toxicity

  • Accumulation toxicity

  • Subchronic toxicity

  • Chronic toxicity

 

The toxicology service offered by Creative Animodel can help you get an in depth understanding of the various types of toxicities. We utilize the latest technologies and state of the art equipments to observe the effects of the chemical on living organisms through various means of administration, and provide an in depth report on the same.

CRISPR-Cas9 Mouse Model used for Genome Editing

The CRISPR-Cas9 is latest popular tool in genome editing. The system’s Cas9 component is an intrinsic part of a newly developed mouse strain. The Cas9 mouse is expected to simplify in vivo genome-editing experiments, particularly those involved in multiple genes and cell types.

The CRISPR/Cas9 system allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The system allows researchers to mutate or change the expression of genes in living cells, including those of humans. The system is analogous to the TALEN DNA nuclease system and can be used to induce mutations at targeted regions in a model organism's genome. 

The Cas9 mouse overcomes packaging size limits. It will not be necessary to set aside valuable space aboard viruses or nanoparticles to convey the bulky Cas9 equipment. Viral or nanoparticlevectors can be packed straightaway with strands of guide RNA, which will also direct Cas9 to DNA targets, and thus it becomes less necessary to manipulate genes one at a time, or to cross animal models to produce strains possessing the desired traits. Besides, it is demonstrated that cells derived from the Cas9 mouse could be extracted for use in lab experiments. Researchers has successfully employed the Cas9-expressing cells removed from the mouse to edit immune dendritic cells , allowing the researchers to experiment with cells that aren't easily accessible and often lack the shelf life to conduct such experiments.

The CRISPR-Cas9 system from bacteria has been applied to genome editing in different species, including Drosophila, elegans , zebrafish , mouse , rat , and also human . The successful correction of disease-causing mutations in animal models via the CRISPR-Cas9 system by researchers in Shanghai Institutes for Biological Scienceshas has demonstrated that the CRISPR-Cas9 system can be also used to cure a genetic disease in mouse by directly correcting the genetic defect through NHEJ or HDR mediated gene editing. It is believed that in the future, there will be more interests in investigating whether similar gene-correction strategies could be used for mutation correction in a setting related to human diseases, such as human stem cells.

It is clear that the CRISPR/Cas system will be widely developed and used as a targeted mutation system in human cells and in mice/rat, due to its superior efficiencies and advantages over similar targeted cleavage systems. As a world leader in custom transgenic animal model, Creative Animodel offers high quality CRISPR/Cas9 System for mutagenesis and genome editing. Creative Animodel has years of experience serving hundreds of clients across six continents. Its full-service model has enabled medical and academic researchers to receive over 1,000 successful and unique gene targeted animal model lines. 

General Toxicology Service at Creative Animodel 

Toxicology is the science dealing with properties, actions, toxicity, fatal dose, detection of, interpretation of the result of toxicological analysis and treatment of poisons. In other words, it is a branch of biology, chemistry, and pharmacology concerned with the study of the adverse effects of chemicals on living organisms. Toxicology test is required by law and the goal of toxicity assessment is to identify adverse effects of a substance.

 

Toxicosis potential is usually affected by multitude of related factors than just by actual toxicity of the toxicant. Exposure-related, biologic, and chemical factors are main factors that influence observed clinical consequences.

 

Each year, there will be numerous new chemicals as well as products of biotechnological industry required for risk assessment. In the area of general toxicology, Creative Animodel offers standard protocols designed to support drug approval submissions to domestic and international regulatory agencies. With multiple test species available, studies are overseen by Study Directors with vast experience gained from performing over 1000 GLP studies. From standard safety studies to more specialized services, Creative Animodel can help clients achieve their study goals more quickly and with greater confidence.

 

Toxicology Areas of Expertise

Acute Toxicity: Acute toxicity exams identify the particular side effects of a material in which effect possibly coming from a one direct exposure or from multiple exposures typically inside of twenty four hours.

 

Accumulation Toxicity: This deposition involving medications along with their particular metabolic byproducts inside parts might be dangerous, bringing about organ harm. Creative Animodel performs typical toxicology methods to evaluate deposition toxicity.

 

Subchronic Toxicity: Research in which go on intended for ninety days or for up to 10% of a examination subject's expected life are thought subchronic. Creative Animodel gives skills inside the planning, design and style, conduct, canceling along with declaring involving subchronic toxicity exams.

 

Chronic Toxicity: Chronic toxicity is often a home of a material which has dangerous effects with a dwelling organism, while in which organism can be subjected to the particular material constantly or repeatedly. Creative Animodel provides AAALAC-accredited features which often are equipped with probably the most innovative technologies to offer customers together with chronic toxicity exams along with help make better drug improvement judgments.

 

 

 

 

RNA Interference for Gene Knockdown and Silence

RNA interference (RNAi) is the process by which the expression of a target gene can be effectively silenced or knocked down. RNA interference involves two types of small ribonucleic acid, namely, microRNA (miRNA) and small interfering RNA (siRNA), which can bind to specific messenger RNA (mRNA) molecules and increase or decrease their activity.

 

RNAi is a latest powerful gene knockdown technique that allows for tissue-specific, temporally controlled suppression of gene expression. Gene knockdown is achieved by causing the destruction of specific mRNA molecules by introducing small double-stranded interfering RNAs (dsRNA) into the cytoplasm. When siRNA is introduced into the cytoplasm of cells, it will be cut into short interfering RNAs (siRNAs) which can target specific mRNA and causing degradation of it. Therefore, the expression of a particular gene can be suppressed by introducing dsRNA whose antisense strand sequence matches the mRNA sequence. Gene knockdown by RNAi is temporary, but this can be solved by transfecting target tissues or cell lines so that they express dsRNA autonomously.

 

The versatility of the technique leads to a number of applications. RNAi can be used in drug or chemical target validation. RNAi can target specific spliced exons, enabling the investigation of the functional roles of alternatively spliced forms of a gene. RNAi is also valuable research tool in cell culture and in living organisms where it can be used for large-scale screens that systematically shut down each gene in the cell, thus helping to identify the components necessary for a particular cellular process or an event.

 

The discovery of RNA interference brings significant advances in both basic research and translational applications. With the elucidation of the RNA interference pathway and the discovery of its participation in crucial biological events, a branch of science has come into being to utilize the RNA interference pathway as a biotechnology for both basic and applied research. To date, small interference RNA, plasmid-, and virus-encoded short-hairpin RNA are regular reagents in laboratories to knockdown the expression of specific genes post-transcriptionally.

Transgenic Animal Models Used in Research

Transgenic animals are organisms with a foreign gene incorporated into their genome, as one of the most important advances in biotechnology. Although knockout animals have been developed for more than twenty years and transgenic mice generation has become quite routine in research labs, many people still do not understand their importance on biomedical research and their capability to improve human health.

 

Many animals share biological similarity with humans. In appearance, humans and animals look quite different, but at a physiological and anatomical level they are remarkably similar. Animals and humans have the same organs and organ systems which perform the same functions in pretty much the same way. Although there are minor differences, the similarities far outweigh those differences. More importantly, these differences can give important clues about diseases and how they might be treated. It has been estimated that rats and mice make up 95% of animals used in research. Humans share approximately 99% of DNA with that of mice.

 

Animal research has become an important subject of research. It has been calculated that there are about 105 Nobel Prizes awarded for Physiology or Medicine in the past, of which 91 directly dependent on animal research. The first Nobel Prize awarded for Physiology or Medicine was given to Emil von Behring in 1901 for developing serum therapy against diphtheria.

 

Why did human need animal’s model for research? In the paper“The Animal Research War” Michael Conn and James Parker have explained: “If you are going to study a human disease you can’t perform the study directly in humans and so you have to develop a model”. The models in vivo are ideal tool and models in living animals are more ideal for investigation. Because of the biological similarity between animals and humans, animal models allow closer approximation to a human response. Animals host different diseases and different responses, while the fundamentals of life are quite the same. Some animals are good human-like models for one thing and some for another; some have a cardiovascular system that is similar to humans while others have similar skin.

 

Transgenic animal is a very useful system for studying mammalian gene function and regulation because analysis is carried out on the whole organism. The main advantage of this technique is the short development time. The complete development of a transgenic mouse line takes about 6 months. There are several professional companies that offer transgenic animal services. The US based company Creative Animodel is a very professional supplier of animal model services. The company provides a complete range of customized transgenic services, which are offered on a modular basis. It has created more than 10,000 founder animals from over 3,500 transgene constructs, with an enviable, overall success rate of 98% in generating transgenic mice/rats/rabbits.

 

General Answers to Questions about Animal Models

Transgenic animal models are a valuable tool for exploring many biological questions, for better understanding diseases, and for producing new drugs to save human lives. It is fair to say that without the use of animal’s models, the possibility of medical breakthrough is significantly reduced. Transgenic animals allow for more efficient scientific advancement due to the inexpensive cost of housing lab animals, their short life cycles, and their rapid reproduction rates.

 

Why are we using animals as models to investigate human diseases?

 

Many animals share biological similarity with humans and the biological similarity of animals   allow closer approximation to a human response. However, animal models of human diseases are only ideal for investigating human disease at a given time. Even though the specific details of how the future therapy are still not clear, each animal model of a condition still furthers basic biological understanding and do contribute a lot to therapeutic advancements.

 

Why don’t we use alternatives to replace animals for research?

 

Some animal protection societies require scientists to reduce, refine and replace the use of animals in research. And there are many methods and technologies invented to replace the use of animals, but completely eliminating animal research is not possible. Techniques, such as computer modeling, micro-dosing, MRI scanning and in vitro testing are boosted as alternatives to the use of live animals. However, every scientific method is designed for a very specific purpose and so those methods cannot replace each other. In vitro experiments, which are done with molecules or cell cultures, are very good to unravel mechanisms that happen inside the cell, but are not always so useful to find out how different tissues and organs interact inside the body. And for all those reasons, we will need to use live animals to answer some of the most important scientific questions related to human health.

 

How we reduce animals’ suffering in labs?

 

Researchers make every effort to minimize animals pain and suffering within labs. On one hand, many of the procedures carried out on animals involve no pain or discomfort, such as observing their behavior or collecting tissue samples after the animal is dead. On the other hand, when some procedures do involve pain or discomfort, some measures will be taken to alleviate their pain, such as using anesthesia and analgesia. Besides, research labs are also trying to provide animals with food, water and a clean, comfortable living environment.

bottom of page